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wild type l tarentolae tar ii  (ATCC)


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    ATCC wild type l tarentolae tar ii
    Growth curves of the L. <t>tarentolae</t> -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)
    Wild Type L Tarentolae Tar Ii, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type l tarentolae tar ii/product/ATCC
    Average 94 stars, based on 25 article reviews
    wild type l tarentolae tar ii - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum"

    Article Title: Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum

    Journal: Iranian Biomedical Journal

    doi: 10.52547/ibj.25.5.349

    Growth curves of the L. tarentolae -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)
    Figure Legend Snippet: Growth curves of the L. tarentolae -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)

    Techniques Used: Cell Culture

    Determination of EGFP expression in CSMF medium in comparison with M199 supplemented with 5% FBS. (A) Assessment of EGFP expression by epifluorescence microscopy, indicating the EGFP expression of L. tarentolae -EGFP and L. tarentolae -PpSP15-EGFP promastigotes in the logarithmic and stationary phases in both CSFM and M199 5% FBS media. (b) Flow cytometry analysis of recombinant L. tarentolae -PpSP15-EGFP (right panel), L. tarentolae EGFP as a positive control (middle panel), and the L. tarentolae wild type as a negative control (left panel) using a FITC detector in CSFM and M199 5% FBS at logarithmic (upper panel) and stationary (lower panel) phases. (C) Western blot analysis to confirm the expression of PpSP15-EGFP by recombinant L. tarentolae -PpSP15-EGFP parasite in CSFM and M199 with 5% FBS using an anti-GFP antibody. A 42-kDa band relating to the expression of PpSP15-EGFP protein in concentrated supernatant of L. tarentolae -PpSP15-EGFP parasite was detected in M199 with 5% FBS (lanes 2 and 3) and CSFM medium (lanes 5 and 6), respectively at logarithmic and stationary phases. A 27-kDa band indicating EGFP protein in the L. tarentolae -EGFP parasite (lane 1) and wild type form of L. tarentolae parasite as negative control was cultivated in M199 with 5% FBS (lane 4)
    Figure Legend Snippet: Determination of EGFP expression in CSMF medium in comparison with M199 supplemented with 5% FBS. (A) Assessment of EGFP expression by epifluorescence microscopy, indicating the EGFP expression of L. tarentolae -EGFP and L. tarentolae -PpSP15-EGFP promastigotes in the logarithmic and stationary phases in both CSFM and M199 5% FBS media. (b) Flow cytometry analysis of recombinant L. tarentolae -PpSP15-EGFP (right panel), L. tarentolae EGFP as a positive control (middle panel), and the L. tarentolae wild type as a negative control (left panel) using a FITC detector in CSFM and M199 5% FBS at logarithmic (upper panel) and stationary (lower panel) phases. (C) Western blot analysis to confirm the expression of PpSP15-EGFP by recombinant L. tarentolae -PpSP15-EGFP parasite in CSFM and M199 with 5% FBS using an anti-GFP antibody. A 42-kDa band relating to the expression of PpSP15-EGFP protein in concentrated supernatant of L. tarentolae -PpSP15-EGFP parasite was detected in M199 with 5% FBS (lanes 2 and 3) and CSFM medium (lanes 5 and 6), respectively at logarithmic and stationary phases. A 27-kDa band indicating EGFP protein in the L. tarentolae -EGFP parasite (lane 1) and wild type form of L. tarentolae parasite as negative control was cultivated in M199 with 5% FBS (lane 4)

    Techniques Used: Expressing, Comparison, Epifluorescence Microscopy, Flow Cytometry, Recombinant, Positive Control, Negative Control, Western Blot



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    Growth curves of the L. <t>tarentolae</t> -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)
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    SDS-PAGE analysis of the level of expression of chimeric sequence that successfully subcloned to pLEXY-neo2 and expressed in Lishmania <t>tarentolae</t> . Lane 1, Protein molecular weight marker (10–140 kDa); Lanes 2: Logarithmic phase secretory sample, Lanes 3: Stationary phase secretory sample, Lanes 4: Logarithmic phase cytosolic sample, Lanes 5: Stationary phase cytosolic sample and Lanes 5: Control ( L. tarentolae secretory sample) (A). Western blot analysis ( B)
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    SDS-PAGE analysis of the level of expression of chimeric sequence that successfully subcloned to pLEXY-neo2 and expressed in Lishmania <t>tarentolae</t> . Lane 1, Protein molecular weight marker (10–140 kDa); Lanes 2: Logarithmic phase secretory sample, Lanes 3: Stationary phase secretory sample, Lanes 4: Logarithmic phase cytosolic sample, Lanes 5: Stationary phase cytosolic sample and Lanes 5: Control ( L. tarentolae secretory sample) (A). Western blot analysis ( B)
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    SDS-PAGE analysis of the level of expression of chimeric sequence that successfully subcloned to pLEXY-neo2 and expressed in Lishmania <t>tarentolae</t> . Lane 1, Protein molecular weight marker (10–140 kDa); Lanes 2: Logarithmic phase secretory sample, Lanes 3: Stationary phase secretory sample, Lanes 4: Logarithmic phase cytosolic sample, Lanes 5: Stationary phase cytosolic sample and Lanes 5: Control ( L. tarentolae secretory sample) (A). Western blot analysis ( B)
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    Image Search Results


    Growth curves of the L. tarentolae -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)

    Journal: Iranian Biomedical Journal

    Article Title: Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum

    doi: 10.52547/ibj.25.5.349

    Figure Lengend Snippet: Growth curves of the L. tarentolae -PpSP15-EGFP parasite cultured in two different media with 1× 10 7 /ml inoculation in 25-cm 2 flasks at 26 °C for a five-day period. (A) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (parasite count/ml) in CSFM medium compared to M199 5% FBS. (B) Growth rate evaluation of L. tarentolae -PpSP15-EGFP parasites (optical density at 600 nm/ml) in CSFM and M199 5% FBS. The experiment was repeated three times, and values are pooled in the Figure. Bars show the mean ± SD. The significant difference in parasite growth rate for different media was determined by t -test analysis and demonstrated as asterisk at the indicated time points ( p < 0.05 denoted as * ). At the time points that are not marked with the asterisk, no significant difference was observed ( p > 0.05)

    Article Snippet: Parasite culture Three types of L. tarentolae , including the wild-type L. tarentolae Tar II (ATCC 30.267) strain, EGFP-expressing L. tarentolae ( L. tarentolae -EGFP) [ ] , and recombinant L. tarentolae -PpSP15-EGFP [ ] (as previously available), as well as wild-type L. major strain (MRHO/IR/75/ER) were used.

    Techniques: Cell Culture

    Determination of EGFP expression in CSMF medium in comparison with M199 supplemented with 5% FBS. (A) Assessment of EGFP expression by epifluorescence microscopy, indicating the EGFP expression of L. tarentolae -EGFP and L. tarentolae -PpSP15-EGFP promastigotes in the logarithmic and stationary phases in both CSFM and M199 5% FBS media. (b) Flow cytometry analysis of recombinant L. tarentolae -PpSP15-EGFP (right panel), L. tarentolae EGFP as a positive control (middle panel), and the L. tarentolae wild type as a negative control (left panel) using a FITC detector in CSFM and M199 5% FBS at logarithmic (upper panel) and stationary (lower panel) phases. (C) Western blot analysis to confirm the expression of PpSP15-EGFP by recombinant L. tarentolae -PpSP15-EGFP parasite in CSFM and M199 with 5% FBS using an anti-GFP antibody. A 42-kDa band relating to the expression of PpSP15-EGFP protein in concentrated supernatant of L. tarentolae -PpSP15-EGFP parasite was detected in M199 with 5% FBS (lanes 2 and 3) and CSFM medium (lanes 5 and 6), respectively at logarithmic and stationary phases. A 27-kDa band indicating EGFP protein in the L. tarentolae -EGFP parasite (lane 1) and wild type form of L. tarentolae parasite as negative control was cultivated in M199 with 5% FBS (lane 4)

    Journal: Iranian Biomedical Journal

    Article Title: Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum

    doi: 10.52547/ibj.25.5.349

    Figure Lengend Snippet: Determination of EGFP expression in CSMF medium in comparison with M199 supplemented with 5% FBS. (A) Assessment of EGFP expression by epifluorescence microscopy, indicating the EGFP expression of L. tarentolae -EGFP and L. tarentolae -PpSP15-EGFP promastigotes in the logarithmic and stationary phases in both CSFM and M199 5% FBS media. (b) Flow cytometry analysis of recombinant L. tarentolae -PpSP15-EGFP (right panel), L. tarentolae EGFP as a positive control (middle panel), and the L. tarentolae wild type as a negative control (left panel) using a FITC detector in CSFM and M199 5% FBS at logarithmic (upper panel) and stationary (lower panel) phases. (C) Western blot analysis to confirm the expression of PpSP15-EGFP by recombinant L. tarentolae -PpSP15-EGFP parasite in CSFM and M199 with 5% FBS using an anti-GFP antibody. A 42-kDa band relating to the expression of PpSP15-EGFP protein in concentrated supernatant of L. tarentolae -PpSP15-EGFP parasite was detected in M199 with 5% FBS (lanes 2 and 3) and CSFM medium (lanes 5 and 6), respectively at logarithmic and stationary phases. A 27-kDa band indicating EGFP protein in the L. tarentolae -EGFP parasite (lane 1) and wild type form of L. tarentolae parasite as negative control was cultivated in M199 with 5% FBS (lane 4)

    Article Snippet: Parasite culture Three types of L. tarentolae , including the wild-type L. tarentolae Tar II (ATCC 30.267) strain, EGFP-expressing L. tarentolae ( L. tarentolae -EGFP) [ ] , and recombinant L. tarentolae -PpSP15-EGFP [ ] (as previously available), as well as wild-type L. major strain (MRHO/IR/75/ER) were used.

    Techniques: Expressing, Comparison, Epifluorescence Microscopy, Flow Cytometry, Recombinant, Positive Control, Negative Control, Western Blot

    SDS-PAGE analysis of the level of expression of chimeric sequence that successfully subcloned to pLEXY-neo2 and expressed in Lishmania tarentolae . Lane 1, Protein molecular weight marker (10–140 kDa); Lanes 2: Logarithmic phase secretory sample, Lanes 3: Stationary phase secretory sample, Lanes 4: Logarithmic phase cytosolic sample, Lanes 5: Stationary phase cytosolic sample and Lanes 5: Control ( L. tarentolae secretory sample) (A). Western blot analysis ( B)

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: In silico analysis and expression of a new chimeric antigen as a vaccine candidate against cutaneous leishmaniasis

    doi: 10.22038/ijbms.2020.45394.10561

    Figure Lengend Snippet: SDS-PAGE analysis of the level of expression of chimeric sequence that successfully subcloned to pLEXY-neo2 and expressed in Lishmania tarentolae . Lane 1, Protein molecular weight marker (10–140 kDa); Lanes 2: Logarithmic phase secretory sample, Lanes 3: Stationary phase secretory sample, Lanes 4: Logarithmic phase cytosolic sample, Lanes 5: Stationary phase cytosolic sample and Lanes 5: Control ( L. tarentolae secretory sample) (A). Western blot analysis ( B)

    Article Snippet: Transfection of pLEXY-TLGL into L. tarentolae The L. tarentolae Tar II (ATCC 30143) strain was grown in RPMI-1640 medium (Gibco) treated with 10% fetal calf serum (FCS, Gibco) affected by heat inactivation (pH: 7.2 and 26 o C).

    Techniques: SDS Page, Expressing, Sequencing, Molecular Weight, Marker, Control, Western Blot